Microscopy, Imaging & Cytometry Resources Core
Room 6339 Scott Hall
Office Fax(313) 577-6739
Dr. Moin is a Professor in the Department of Pharmacology and the Director of the Microscopy, Imaging and Cytometry Resources (MICR) Core, a university-wide service facility. He is an internationally recognized leader in the field of optical imaging and has been heavily involved in development and testing of functional optical probes and assays for live cell imaging as well as in vivo small animal imaging with the potential for translation into the clinical settings.
Dr. Moin's primary research interest has been the role proteases and their inhibitors may play in tumor progression and metastasis. As such he has been studying the intracellular expression and distribution of the cysteine protease cathepsin B and cysteine protease inhibitors (CPIs) in tumors and tumor cells, both in vivo and in vitro. Initially the research focused on the characterization of cathepsin B and the CPIs at the molecular and cellular levels. Dr. Moin and his colleagues had shown that there was a fundamental shift in the trafficking of the protease to the tumor cell membrane and its subsequent secretion into the extracellular milieu that may have direct effect in tumor progression and metastasis. To expand and further these efforts, in recent years the research focus has diverged toward functional imaging of proteases in tumor and tumor microenvironment to complement and enhance the ongoing molecular characterization studies. The primary research tools for these endeavors have been molecular imaging techniques including fluorescent, confocal and multiphoton microscopy as well as small animal optical imaging.
Dr. Moin is the Founding Director of MICR, a service facility providing conventional, fluorescent, confocal and multi-photon microscopy as well as Flow Cytometry and Animal Imaging instrumentation and expertise.
B.S., University of Minnesota, Minneapolis, MN, 1977, Zoology
M.S., University of Wisconsin, LaCrosse, WI, 1980, Biology
Ph.D., University of Montana, Missoula, MT, 1986, Microbiology
Postdoctoral Fellowship, Wayne State University School of Medicine, Detroit, MI, 1986-1990
Areas of Expertise
Microscopy and Imaging
Areas of Interest
Role of Proteases in Cancer Progression
- Moin, K., Sameni, M., Victor, B.C., Rothberg J.M., Mattingly, R.R. and Sloane, B.F. 3D/4D functional imaging of tumor-associated proteolysis: impact of microenvironment. Meth. Enzymol. 506:175-94, 2012.
- Sameni, M., Anbalagan, A., Olive, M. B., Moin, K., Mattingly, R. R., Sloane, B. F. MAME Models for 4D Live-cell Imaging of Tumor:Microenvironment Interactions that Impact Malignant Progression. J. Vis. Exp. (60), e3661, DOI: 10.3791/3661, 2012. PMC3845223
- Withana NP, Blum G, Sameni M, Slaney C, Anbalagan A, Olive MB, Bidwell BN, Edgington L, Wang L, Moin K, Sloane BF, Anderson RL, Bogyo MS, Parker BS. Cathepsin B inhibition limits bone metastasis in breast cancer. Cancer Res. 2012. PMID: 22266111
- Moin, K., Sameni, M., Victor, B.C., Rothberg J.M., Mattingly, R.R. and Sloane, B.F. 3D/4D functional imaging of tumor-associated proteolysis: impact of microenvironment. Meth. Enzymol. 506:175-94, 2012. PMID: 22341225.
- Mullins, S.R., Sameni, M., Blum, G., Bogyo, M., Sloane, B.F. and Moin, K. Three-dimensional cultures modeling premalignant progression of human breast epithelial cells: role of cysteine cathepsins. Biol. Chem. 393: 1405-1416, 2012. PMID: 23087100
- Rothberg JM, Bailey KM, Wojtkowiak JW, Ben-Nun Y, Bogyo M, Weber E, Moin K, Blum G, Mattingly RR, Gillies RJ, Sloane BF. Acid-mediated tumor proteolysis: contribution of cysteine cathepsins. Neoplasia. 10:1125-37, 2013. PMID: 24204192.
- Webber E, Barbulescu E, Medek R, Reinheckel T, Sameni M, Anbalagan A, Moin K, Sloane BF. Cathepsin B-deficient mice as source of monoclonal anti-cathepsin B antibodies. Biol. Chem. 395: aop, 2014. PMID: 25205719
- Ramalho SD, Sharma R, White JK, Aggarwal N, Chalasani A, Sameni M, Moin K, Vieira PC, Turro C, Kodanko JJ, Sloane BF. Imaging Sites of Inhibition of Proteolysis in Pathomimetic Human Breast Cancer Cultures by Light-Activated Ruthenium Compound. PLoS One. 10(11):e0142527. doi: 10.1371/journal.pone.0142527, 2015. PMID: 26562785
- Chalasani, A., Ji, K., Sameni, M., Mazumder, S., Xu, Y., Moin, K., Sloane, B.F. Live-Cell Imaging of Protease Activity: Assays to Screen Therapeutic Approaches. Methods Mol Biol. (1574): 215-225, 2017. PMCID: PMC5500169
- Cavallo-Medved D, Sloane BF, Moin K. Cathepsin B. Encyclopedia of Signaling Molecules. Springer and Cham; 2018. p. 746–762.